Asian Journal of Bio Science

Shaheen Jamal ¹, Sunita ²

Affiliations
1 Department of Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, LUDHIANA (PUNJAB), IN
2 Krishi Vighan Kendra, Budhsinghwala, MOGA (PUNJAB), IN

Abstract

Cellulases are used in numerous industrial applications and for cellulose conversion to value added products. A cellulase producing thermophilic Aspergillus fumigatus was isolated from paper industry effluent sample after 72 h incubation on potato dextrose agar at 45°C. In the present study the production of cellulase enzyme by Aspergillus fumigatus cultivated on wheat bran: rice straw (1:4) using solid state fermentation (SSF) technique. The ability to produce cellulase under varying conditions of temperature, pH, moisture content, and nitrogen sources was evaluated. The higher cellulase activity was obtained when the fungus was cultivated on substrate (wheat bran: rice straw (1:4)) with moisture content (1:4), pH 5.5, urea as nitrogen source and incubated in environmental chamber at 45°C for 3 days as it give 1.85FPU/ml.

Keywords

Cellulase, Thermophilic, Aspergillus fumigates, Paper Industry, Solid State Fermentation.

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Sunita ¹

Affiliations
1 Krishi Vigyan Kendra, Budhsinghwala, Moga (Punjab), IN

Abstract

Vegetables included in daily schedule of diet viz., sweet pepper, cauliflower, carrot, cabbage, lettuce, spinach, tomato, potato, reddish, and bottle gourd are good for human health. Vegetables intake is beneficial for obese as they furnish fat to a lesser extent. The determination of nutrient analysis (total protein, fat, carbohydrate, ash, energy value and moisture content) of vegetables is important to plan the diet for various therapeutic purposes. Macro nutrients viz., calcium (Ca), magnesium (Mg), potassium (K), sodium (Na), and phosphorus (P) and micro nutrients viz., iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), nickel (Ni) and selenium (Se) elements have importance for the several purposes like formation of blood, bones and teeth even other tissues, osmoregulation of body fluids, control of physico-chemical process etc. This paper elaborates the various methods used to determine the nutrient profile and mineral analysis of vegetables in detail.

Keywords

Vegetables, Human Health, Nutrient Profile, Minerals.

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Shaheen Jamal ¹, Sunita ¹

Affiliations
1 Department of Biotechnology, Lovely Professional University, Phagwara (Punjab), IN

Abstract

Membrane proteins perform diverse functions in living organisms such as transporters, receptors and channels. The functions of membrane proteins have been investigated with several computational approaches, such as developing databases, analyzing the structure function relationship and establishing algorithms to discriminate different type of membrane proteins. However, compilation of bioinformatics resources for the functions of membrane proteins is not well documented compared with their structural aspects. The purpose of the present work was to assess the study of transmembraneous protein using bioinformatics and data mining. Bioinformatics is the application of information technology to the field of molecular biology. Protein structure prediction is the important application of bioinformatics. Bioinformatics also provide researchers with software's and tools for analyzing the sequence data and deriving biologically meaningful information from a string of letters. By using application of bioinformatics one can predict isoelectric point, molecular weight, transmembrane helix and secondary structure of transmembrane protein.

Keywords

Membrane Proteins, Bioinformatics, Data Mining, Transmembrane Helix.

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